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Series GSE71426 Query DataSets for GSE71426
Status Public on Jul 29, 2017
Title MHC Class I antibody induced gene expression in mouse obliterative airway disease
Organism Mus musculus
Experiment type Expression profiling by array
Summary To further understand the early molecular events in antibody mediated chronic inflammation of the lungs, we utilized a murine Obliterative Airway Disease (OAD) model of native lungs where Abs to MHC class I induces bronchiolar obliteration and fibrosis. This study identified a unique transcriptional profile with a set of genes that were stimulated while another set of genes that were repressed in anti MHC I treated lungs compared to that of isotype treated lungs.
Lung transplantation (LTx) is an accepted clinical intervention for various intractable pulmonary dysfunctions. However, de novo Abs to mismatched donor HLA (DSA) predispose lung-grafts to chronic rejection, Bronchiolitis Obliterans Syndrome (BOS). The anti-MHC induced mouse OAD model, the only available preclinical model for study of early pathogenesis following MHC ligation, produces lesions analogous to BOS.
Overall design Induction of gene expression was measured following intrabronchial administration of anti-MHC class I (H-2Kb) Ab at 200μg/mouse. Samples were collected on days 1, 2, 3 and 6 post-Ab administration. Isotype matched control Ab was administered at an equivalent dose and gene expression profile in isotype treated group served as the base line expression and was compared with that of anti-H-2Kb treated group. A total of 24 samples were processed with two treatment groups (anti-H-2Kb, isotype), four time points (days 1, 2, 3 and 6) and three biological replicates.
Contributor(s) Nayak DK, Zhou F, Huang J, Tsuji M, Yu J, Hachem R, Gelman A, Bremner R, Xu M, Smith M, Mohanakumar T
Citation(s) 28701473
Submission date Jul 28, 2015
Last update date Nov 01, 2017
Contact name Deepak K Nayak
Organization name University of Arizona College of Medicine
Street address 475 N. 5th Street
City Phoenix
State/province Arizona
ZIP/Postal code 85004
Country USA
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (24)
GSM1834070 Lung, isotype, day 1, rep 1
GSM1834071 Lung, isotype, day 1, rep 2
GSM1834072 Lung, isotype, day 1, rep 3
BioProject PRJNA291185

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71426_RAW.tar 218.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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