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Series GSE7102 Query DataSets for GSE7102
Status Public on Feb 21, 2008
Title siRNA-mediated knockdown of Sp3 induces a pro-inflammatory pattern of cytokine production: relevance to MS
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Multiple sclerosis (MS) is an inflammatory disease of the central nervous system and is generally considered to be autoimmune in nature. We previously demonstrated that the transcription factor Sp3 is significantly down-regulated in immune cells from MS patients. The potential role of Sp3 down-regulation in MS pathogenesis is not well understood. The function of endogenous Sp3 was assessed in vitro after siRNA-mediated knockdown of its transcript in Jurkat cells. Sp3 protein levels were reduced an average of 70%. ELISA studies demonstrated decreased endogenous production of IL-10 and TGFβ1 and increased endogenous production of TNFα (p<0.05 in all assays). Subsequent microarray analysis demonstrated significantly altered expression of 36 genes (p<0.001 for each gene) compared with control samples. Analysis showed differential expression (p<0.005) of 8 gene pathways. Many of the genes and pathways that were regulated by Sp3 are involved in immune function, specifically with regard to apoptosis, cell-to-cell adhesion, integrin signaling, T-cell differentiation, and cytokine production. This study identifies mechanisms by which Sp3 may regulate immune function and suggests a basis for its potential contribution to MS disease pathogenesis.
Keywords: siRNA knockdown; Jurkat T-cells; Multiple Sclerosis
 
Overall design Two Sp3 siRNA knockdown groups (siRNA Sp3 #2: n=3, and #6: n=3) were clustered together into the Treated condition, and the two control groups (siRNA for GAPDH: n=3, and Non-transfected cells: n=3)were assembled to constitute the Control condition. Thus a total of 12 arrays (6 Controls and 6 treated) were used in this experiment.
 
Contributor(s) Schwechter AD, Grekova M, Ressom HW, Notario V, Richert JR
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Submission date Feb 21, 2007
Last update date Dec 06, 2018
Contact name Azik Schwechter
E-mail(s) schwechtera@mail.nih.gov
Organization name NIH
Department NIMH
Lab Labratory fo Molecular Biology
Street address NIH/NIMH 10 Center Drive Room 4D/14
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (12)
GSM170817 Control 1
GSM170818 Control 2
GSM170821 Control 3
Relations
BioProject PRJNA98443

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7102_RAW.tar 59.3 Mb (http)(custom) TAR (of CEL, CHP, EXP)
Processed data provided as supplementary file

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