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Series GSE70947 Query DataSets for GSE70947
Status Public on Jul 01, 2016
Title Age and estrogen-dependent inflammation in breast adenocarcinoma and normal breast tissue [cohort_2]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Chronic inflammation promotes breast tumor growth and invasion by accelerating angiogenesis and tissue remodeling in the tumor microenvironment. The relationship between inflammation and estrogen, which drives the growth of 70 percent of breast tumors, is complex. Low levels of estrogen exposure stimulate macrophages and other inflammatory cell populations, but very high levels are immune suppressive. Breast tumor incidence is increased by obesity and age, which interact to influence inflammatory cell populations in normal breast tissue. The molecular impact of these factors on tumor initiation and growth is not well-understood. We modeled the difference in gene expression between 195 breast adenocarcinomas and 195 matched adjacent normal breast tissue samples, using age, body mass index (BMI), and tumor subtype as covariates. Age and BMI were independently associated with inflammation in normal tissue but not tumors. Older patients with ER-positive disease had tumors with higher levels of Estrogen Receptor (ER) signaling compared to adjacent normal tissue and had lower relative levels of tumor macrophage expression. We developed a novel statistic to quantify the rewiring of gene co-expression networks and demonstrate that in ER-positive tumors basal gene networks are rewired even though their expression levels of these genes are not significantly different from those in adjacent normal tissue. Patient age influences the molecular profile of ER-positive breast tumors. Our data support an immunosuppressive effect of estrogen signaling in the breast tumor microenvironment, suggesting this effect contributes to the greater presence of prognostic and therapeutically relevant immune cells in ER-negative tumors.
Overall design 296 total samples: 148 breast adenocarcinoma, 148 paired adjacent normal breast tissue
Contributor(s) Quigley DA, Kristensen V
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Submission date Jul 15, 2015
Last update date Nov 27, 2018
Contact name David Quigley
Organization name UCSF
Department Helen Diller Comprehensive Cancer Center
Lab Ashworth Lab
Street address 1450 Third St. Room 207
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (296)
GSM1823702 CM016-normal
GSM1823703 CM022-normal
GSM1823704 CM082-normal
This SubSeries is part of SuperSeries:
GSE70951 Paired Breast Adenocarcinoma and Adjacent Normal tissue
BioProject PRJNA290007

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70947_RAW.tar 3.6 Gb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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