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Series GSE70930 Query DataSets for GSE70930
Status Public on Aug 06, 2015
Title Single-cell RNA-seq of bone marrow-derived mesenchymal stem cells reveals unique profiles of lineage priming
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The plasticity and immunomodulatory capacity of mesenchymal stem cells (MSCs) have spurred clinical use in recent years. However, clinical outcomes vary and many ascribe inconsistency to the tissue source of MSCs. Yet unconsidered is the extent of heterogeneity of individual MSCs from a given tissue source with respect to differentiation potential and immune regulatory function. Here we use single-cell RNA-seq to assess the transcriptional diversity of murine mesenchymal stem cells derived from bone marrow. We found genes associated with MSC multipotency were expressed at a high level and with consistency between individual cells. However, genes associated with osteogenic, chondrogenic, adipogenic, neurogenic and vascular smooth muscle differentiation were expressed at widely varying levels between individual cells. Further, certain genes associated with immunomodulation were also inconsistent between individual cells. Differences could not be ascribed to cycles of proliferation, culture bias or other cellular process, which might alter transcript expression in a regular or cyclic pattern. These results support and extend the concept of lineage priming of MSCs and emphasize caution for in vivo or clinical use of MSCs, even when immunomodulation is the goal, since multiple mesodermal (and even perhaps ectodermal) outcomes are a possibility. Purification might enable shifting of the probability of a certain outcome, but is unlikely to remove multilineage potential altogether.
Overall design Examination was performed using single-cell RNA-seq of sixteen mouse MSCs (mMSC1-mMSC16), non MSC single cell controls (HL1cm1-HL1cm5), and the population controls (mMSC-PC and HL1cm-PC).
Contributor(s) Freeman BT, Jung PJ, Ogle BM
Citation(s) 26352588
Submission date Jul 15, 2015
Last update date May 15, 2019
Contact name Brenda M Ogle
Organization name University of Minnesota-Twin Cities
Department Biomedical Engineering
Lab Systems Regeneration Laboratory
Street address 312 Church St SE
City Minneapolis
State/province Minnesota
ZIP/Postal code 55455
Country USA
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (23)
GSM1822022 mMSC1
GSM1822023 mMSC2
GSM1822024 mMSC3
BioProject PRJNA289962
SRA SRP061185

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Supplementary file Size Download File type/resource
GSE70930_Freeman_Processed_Data_PLOSone.txt.gz 777.5 Kb (ftp)(http) TXT
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