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Series GSE70800 Query DataSets for GSE70800
Status Public on Jul 13, 2016
Title Molecular signatures of neural connectivity in the olfactory cortex
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose:This work aimed to identify the genetic profiles of piriform projection neurons and characterize their spatial organization within the piriform cortex.
Methods: We microdissected the three layers of pirifrom cortex by laser capture (LMD) and performed RNA deep sequencing in order to identify layer-specific molecular markers, we then validated these data by using RNA in situ hybridization and immunohistochemistry.We next performed anterograde neural tracing experiments to identify piriform target regions, and retrograde neural tracing experiments to analyze how piriform projection neurons are organized within piriform cortex.We then combined the analysis of patterns of gene expression with retrograde tracing experiments to identify molecular signatures of the different subclasses of piriform projecting neurons.
Results:We show that layers and sub-layers of the piriform cortex can be discriminated by gene expression patterns in adult piriform cortex. We observe that neurons projecting to distinct target areas are localized in distinct layers and express specific genes. We demonstrate that these molecular signatures of piriform projection neurons are maintained in reeler mice, in which cortical lamination is lost and neural positioning is scrambled, suggesting that piriform output connectivity strictly depends on the molecular programm, rather than a proper lamination of the cortex.
Conclusion:These results provide important insights into the principles underling the piriform connectivity.
Overall design 3 replicates per each layer (three layers) of piriform cotrex were used for the RNA deep sequancing
Contributor(s) Diodato A, Fleischmann A, Pouch J, Perrin S
Citation(s) 27426965
Submission date Jul 11, 2015
Last update date May 15, 2019
Contact name Stephane Le Crom
Organization name IBENS
Department Genomic Core Facility
Street address 46 rue Ulm
ZIP/Postal code 75230
Country France
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (9)
GSM1819200 Layer A1
GSM1819201 Layer A2
GSM1819202 Layer A3
BioProject PRJNA289565
SRA SRP060674

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Supplementary file Size Download File type/resource
GSE70800_RAW.tar 3.3 Mb (http)(custom) TAR (of TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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