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Series GSE70694 Query DataSets for GSE70694
Status Public on Jul 15, 2015
Title Cas9 gRNA engineering for selectable genome editing, activation and repression
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary RNA-Seq after Cas9-gRNA transfection with different length gRNAs
Overall design we performed PolyA Selection and RNA-Seq on cells transfected with dCas9-VPR and a gRNA of each length (20nt, 16nt, or 14nt) targeting ACTC1, MIAT, or HBG1/2
Contributor(s) Chavez A, Ter-Ovanesyan D
Citation(s) 26344044
Submission date Jul 09, 2015
Last update date May 15, 2019
Contact name George Chruch
Organization name Harvard Medical School
Department Genetics
Street address 77 Ave Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (24)
GSM1816572 ACTC1_VPR 20nt Rep1
GSM1816573 ACTC1_VPR 20nt Rep2
GSM1816574 ACTC1_VPR 16nt Rep1
BioProject PRJNA289388
SRA SRP060637

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70694_RAW.tar 15.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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