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Series GSE70627 Query DataSets for GSE70627
Status Public on Mar 21, 2016
Title Notch1 Signaling Regulates the Aggressiveness of Differentiated Thyroid Cancer and Inhibits SERPINE1 Expression
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To identify novel down-stream effectors of Notch1 signaling, we have employed cDNA microarray expression profiling as a discovery platform. Human follicualr thyroid carcinoma cell line FTC236 was engineered with tet-on inducible system to establish a stable cell line. The stable cell line FTC236-Notch1 showed robust inducibility with doxycycline, which triggers the expression of Notch1 intracellular domain (NICD). The mRNA samples from FTC236-Notch1 cells with or without doxycline treamtment were analyzed in this assay. Top 50 gene sequences were identified as of the most significance which distinguished between doxycycline treated samples and control samples. Expression of SERPINE1, a down-regulated gene duing Notch1 activation from this signature, was quantified in the same RNA samples by real-time PCR. The protein level was further confirmed by Western blot that restoration of NICD inhibits the expression of SERPINE1.
Overall design NICD induced gene expression in human follicular thyroid cancer cell line was measured at 48 hours after exposure to doses of 0, and 1 ug/mL of doxycycline. Three independent experiments were performed at separate time.
Contributor(s) Johnson BP, Yu X, Chen H
Citation(s) 26847059
Submission date Jul 08, 2015
Last update date Nov 27, 2018
Contact name Brian P Johnson
Phone 608-262-1209
Organization name UW-Madison
Department Oncology
Lab Chris Bradfield
Street address 1400 University Ave
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (6)
GSM1812078 FTC236 Notch1_48hr_1ugDox_rep1
GSM1812079 FTC236 Notch1_48hr_1ugDox_rep2
GSM1812080 FTC236 Notch1_48hr_1ugDox_rep3
BioProject PRJNA289234

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Supplementary file Size Download File type/resource
GSE70627_RAW.tar 80.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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