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Series GSE70307 Query DataSets for GSE70307
Status Public on Jun 26, 2016
Title global gene expression profiling experiments of HSC cultured in cysteine or valine deficient medium by RNA-sequencing analysis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We investigated the role of amino acid to maintain HSC function. To identify essential amino acids for HSCs, CD34-KSL cells were cultured in single amino acids deficient medium. And cultured cells were transplanted into lethally irradiated mice. Then, the donor chimerism and lineage contribution was estimated. Surprisingly, HSC proliferation was prevented in valine and cysteine deficient medium in vitro. Donor cells cultured in these medium were also not engrafted. To elucidate the effects and influences of cysteine and valine in HSCs, we performed global gene expression profiling experiments by RNA-sequencing analysis. Gene sets categorized with cell cycle, mitosis, cell division or DNA replication were strongly down-regulated in both valine- or cysteine-depleted conditions These results imply distinctive amino-acid metabolism involved in HSC division.
Overall design Gene expression profiles of ten thousand HSCs cultured in cysteine or valine deficient medium for 24 hours were compared with that of HSCs cultured in complete medium by using RNA-sequencing analysis
Contributor(s) Taya Y, Yamazaki S, Watarai H, Kanazawa A, Endo TA
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Submission date Jun 26, 2015
Last update date May 15, 2019
Contact name Takaho A. Endo
Organization name RIKEN
Department IMS
Lab Laboratory for Integrative Genomics
Street address 1-7-22 Suehiro, Tsurumi
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (3)
GSM1723515 CD34- HSC cultured under complete medium
GSM1723516 CD34- HSC cultured under medium depleted of valine
GSM1723517 CD34- HSC cultured under medium depleted of cystein
BioProject PRJNA288290
SRA SRP059910

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Supplementary file Size Download File type/resource
GSE70307_RAW.tar 380.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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