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Series GSE70299 Query DataSets for GSE70299
Status Public on Oct 14, 2016
Title The evolution of N6-methyladenosine in primates
Organisms Macaca mulatta; Pan troglodytes; Homo sapiens
Experiment type Other
Summary Phenotypic differences within populations and between closely related species are often driven by variation and evolution of gene expression. However, most analyses have focused on the effects of genomic variation at cis-regulatory elements such as promoters and enhancers that control transcriptional activity, and little is understood about the influence of post-transcriptional processes on transcript evolution. Post-transcriptional modification of RNA by N6-methyladenosine (m6A) has been shown to be widespread throughout the transcriptome, and this reversible mark can affect transcript stability and translation dynamics. Here we analyze m6A mRNA modifications in lymphoblastoid cell lines (LCLs) from human, chimpanzee and rhesus, and we identify patterns of m6A evolution among species. We find that m6A evolution occurs in parallel with evolution of consensus RNA sequence motifs known to be associated with the enzymatic complexes that regulate m6A dynamics, and expression evolution of m6A-modified genes occurs in a parallel evolutionary pattern with m6A evolution. Further, genes modified by evolved m6A in humans are significantly enriched in transcriptional regulatory processes and disease related pathways, suggesting an important role in human biology for the evolution of m6A modification.

Overall design Perform m6A-seq in human, chimpanzee and rhesus, and identify m6A evolutionary mode in primates.
Contributor(s) Ma L, White KP
Citation(s) 28052920
Submission date Jun 26, 2015
Last update date May 15, 2019
Contact name Kevin P. White
Organization name University of Chicago
Department Institute for Genomics and Systems Biology
Street address 900 E. 57th STR. 10th FL.
City Chicago
State/province IL
ZIP/Postal code 60615
Country USA
Platforms (3)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL19129 Illumina HiSeq 2500 (Macaca mulatta)
GPL19148 Illumina HiSeq 2500 (Pan troglodytes)
Samples (16)
GSM1723347 H1_IP
GSM1723348 H1_input
GSM1723349 H2_IP
BioProject PRJNA288267
SRA SRP059908

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70299_RAW.tar 540.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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