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Series GSE70176 Query DataSets for GSE70176
Status Public on Jan 15, 2016
Title Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy [1]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Focal amplifications of 3p13-3p14 occur in about 10% of melanoma and are associated with poor prognosis. The melanoma-specific oncogene MITF resides at the epicenter of this amplicon1. However, whether other loci present in this amplicon also contribute to melanomagenesis is unknown. Here we show that the recently annotated long non-coding RNA gene LINC01212 is consistently co-gained with MITF. In addition to being amplified, LINC01212 is a target of the lineage-specific transcription factor SOX10 and, consequently, it is expressed in more than 90% of human melanomas, but not in normal adult tissues. Whereas exogenous LINC01212 functions in trans to increase melanoma clonogenic potential LINC01212 knock-down dramatically decreases the viability of melanoma cells irrespective of their transcriptional cell state, BRAF, NRAS or TP53 status, diminishes melanoma growth and increases their sensitivity to MAPK-targeting therapeutics both in vitro and in patient-derived melanoma xenograft mouse models. Mechanistically, LINC01212 functions as a lineage addiction oncogene by interacting with and modulating the cellular localization and function of two proteins, XRN2 and p32, involved in nucleolar and mitochondrial rRNA processing, ribosome biogenesis and protein synthesis. LINC01212 targeting, especially in combination with BRAFV600E-inhibitors, is expected to deliver highly effective and tissue-restricted antimelanoma therapeutic responses.
Overall design LINC01212 knockdown or overexpression in 4 and 1 cell line respectively. Depending on the cell line, 3-4 replicates per condition are included.
Contributor(s) Mestdagh P, Vandesompele J, Leucci E, Marine J
Citation(s) 27008969
Submission date Jun 23, 2015
Last update date Apr 04, 2016
Contact name Pieter mestdagh
Organization name Center for Medical Genetics
Street address De Pintelaan 185
City Gent
ZIP/Postal code 9000
Country Belgium
Platforms (1)
GPL20604 Agilent-041648 LincRNA 60K Microarray PVD version 2 (probe name version)
Samples (38)
GSM1717959 MM087 control rep1
GSM1717960 MM087 control rep2
GSM1717961 MM087 control rep3
This SubSeries is part of SuperSeries:
GSE70180 Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy
BioProject PRJNA287797

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70176_RAW.tar 123.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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