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Series GSE70050 Query DataSets for GSE70050
Status Public on Dec 31, 2016
Title Lack of allergy to timothy grass pollen is not a passive phenomenon but associated with allergen specific modulation of immune reactivity
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Timothy grass (TG) pollen is a common seasonal airborne allergen associated with symptoms ranging from mild rhinitis to severe asthma. The aim of this study was to characterize changes in TG-specific T cell responses as a function of seasonality. Peripheral blood mononuclear cells (PBMC) obtained either during the pollen season or out of season, from allergic individuals and non-allergic controls were stimulated either with TG extract or a pool of previously identified immunodominant antigenic regions. PBMC from in season allergic subjects exhibit higher IL-5 and IL-10 responses compared to out of season donors. In the case of non-allergic subjects, as expected we observed lower IL-5 responses and robust production of IFN╬│ compared to allergic individuals. Strikingly, non-atopic donors exhibited an opposing pattern with decreased immune reactivity in-season. The broad downregulation in non-allergic donors indicates that healthy individuals are not oblivious to allergen exposure but rather react with an active modulation of the responses following the antigenic stimulus provided during the pollen season. Transcriptomic analysis of allergen-specific T cells defined genes modulated in concomitance with allergen exposure and inhibition of responses in non-allergic donors. Magnitude and functionality of T-helper cell responses differ substantially for in season versus out of season in allergic and non-allergic subjects. The results indicate specific and opposing modulation of immune responses following the antigenic stimulation during the pollen season. This seasonal modulation reflects the enactment of specific molecular programs associated with health and allergic disease.
 
Overall design 11 allergen-specific T cell RNA samples were analyzed: 5 isolated from PBMC of allergic individuals and 6 from non-allergic individuals (considered as the control group).
 
Contributor(s) Hintz D, Seumois G, Gholami AM, Greenbaum JA, Lane J, White B, Peters B, Vijayanand P, Sette A
Citation(s) 26662458
Submission date Jun 19, 2015
Last update date Jul 24, 2019
Contact name Alessandro Sette
E-mail(s) alex@lji.org
Organization name La Jolla Institute for Allergy and Immunology
Street address 9420 Athena Circle
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (76)
GSM1868722 RNA-Seq_ALL01_IN_TCM_IL5
GSM1868723 RNA-Seq_ALL01_IN_TEM_IFNG
GSM1868724 RNA-Seq_ALL01_IN_TCM_IFNG
Relations
BioProject PRJNA287538
SRA SRP059689

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70050_All_Count.tsv.gz 1.3 Mb (ftp)(http) TSV
GSE70050_All_Counts_DESeq_normalized.csv.gz 5.1 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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