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Series GSE69877 Query DataSets for GSE69877
Status Public on Sep 28, 2017
Title MCPyV ST, MAX and the TRRAP complex cooperate to bind to Transcription Start Sites
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To determine if MCPyV ST was recruited to chromatin together with MAX and the TRRAP complex, we performed chromatin immunoprecipitation (ChIP) using the validated antibodies to MCPyV ST produced in our lab, HA tagged ST, MAX and EP400 followed by next generation sequencing. De novo DNA motif analysis revealed that the canonical E-box MYC target sequence was the most frequently observed motif. Metagene analysis revealed that antibodies to MAX, EP400, ST (Ab5) and HA tagged ST showed strong enrichment in transcription start site (TSS). H3K4me3 ChIP-seq confirmed that the peaks enriched with antibodies to MAX, EP400 and ST all centered on the H3K4me3 peaks with a high degree of overlap.
Overall design Examination of the hypothesis that transcripton factors Max and TRRAP complex bind same DNA loci as a complex.
Contributor(s) Cheng J, Zeid R, Westerling T
Citation(s) 29028833
Submission date Jun 15, 2015
Last update date May 15, 2019
Contact name Jingwei Cheng
Phone 617-632-4797
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Lab James A. DeCaprio
Street address 450 Brookline Ave
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (10)
GSM1711855 Input DNA rep1
GSM1711856 ST-HA_ChIP-seq
GSM1711857 EP400_ChIP-seq rep1
This SubSeries is part of SuperSeries:
GSE69878 MCPyV ST activates Max target genes by recruiting TRRAP/EP400 complex
BioProject PRJNA287016
SRA SRP059497

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69877_MAX.peaks_bothreps.bed.gz 1.3 Mb (ftp)(http) BED
GSE69877_MAX.peaks_bothreps.txt.gz 2.4 Mb (ftp)(http) TXT
GSE69877_RAW.tar 70.1 Mb (http)(custom) TAR (of BED, TXT, WIG)
GSE69877_ST.peaks_bothreps.bed.gz 336.0 Kb (ftp)(http) BED
GSE69877_ST.peaks_bothreps.txt.gz 590.3 Kb (ftp)(http) TXT
GSE69877_p400.peaks_bothreps.bed.gz 549.1 Kb (ftp)(http) BED
GSE69877_p400.peaks_bothreps.txt.gz 993.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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