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Series GSE69839 Query DataSets for GSE69839
Status Public on May 24, 2016
Title CENP-T and CENP-C bridge adjacent CENP-A nucleosomes on young alpha-satellite dimers
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We find that CENP-T acts as a bridge between two well-positioned CENP-A nucleosomes that are present on young alpha-satellite dimers that dominate functional human centromeres. CENP-T is centered over the CENP-B box, where it interacts with the CENP-B/CENP-C complex. Upon cross-linking, the entire CENP-A/CENP-C/CENP-T-containing complex is recovered as a nuclease-protected particle over an alpha-satellite dimer that comprises the fundamental unit of kinetochore chromatin. Our work reveals that CENP-A/CENP-C and CENP-T branches of kinetochore assembly are physically integrated.
 
Overall design We used chromatin immunoprecipitation with sequencing (ChIP-seq) in human cells to determine the relative positions of CENP-A and CENP-T particles at functional centromeres.
 
Contributor(s) Thakur J, Henikoff JG, Henikoff S
Citation(s) 27384170
Submission date Jun 12, 2015
Last update date May 15, 2019
Contact name Jorja Henikoff
E-mail(s) jorja@fhcrc.org
Phone 206-667-4850
Organization name Fred Hutchinson Cancer Research Center
Department Basic Sciences
Lab Henikoff
Street address 1100 Fairview AV N, A1-162
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (30)
GSM1709883 HuRef_CenpT_1_(20150213_1_6)
GSM1709884 HuRef_CenpT_2_(20150213_1_12)
GSM1709885 HuRef_CenpT_3_(20150213_1_19)
Relations
BioProject PRJNA286896
SRA SRP059445

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69839_Cen1-like.fa.gz 326 b (ftp)(http) FA
GSE69839_Cen13-like.fa.gz 375 b (ftp)(http) FA
GSE69839_RAW.tar 8.7 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record
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