|
| Status |
Public on Jun 10, 2017 |
| Title |
RNA-seq profiling of cortical collecting ducts in rats undergoing vasopressin escape |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We sequenced mRNAs in cortical collecting ducts microdissected from rats undergoing vasopressin escape.
|
| |
|
| Overall design |
Rats were given subcutaneous DDAVP for 5 days via subcutaneous osmotic minipumps. Rats in treatment group (n=3-4) were given high water load (50 mL/day) and rats in control group were given normal water load (25 mL/day). After 1, 2, and 4 days, rats were sacrificed and cortical collecting ducts were microdissected and collected from collagenase-digested left kidney for RNA-seq profiling of mRNAs.
|
| |
|
| Contributor(s) |
Lee JW, Ecelbarger CM, Knepper MA |
| Citation(s) |
28843412 |
| |
| Submission date |
Jun 11, 2015 |
| Last update date |
May 23, 2023 |
| Contact name |
Jae Wook Lee |
| E-mail(s) |
leejw4@mail.nih.gov
|
| Organization name |
National Institutes of Health
|
| Street address |
10 Center Drive, 6N314
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
|
| Samples (20)
|
|
| Relations |
| BioProject |
PRJNA286770 |
| SRA |
SRP059370 |
Less...
More...