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Series GSE69726 Query DataSets for GSE69726
Status Public on Dec 02, 2015
Title Myocardin-related transcription factors are required for cardiac development and function
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Myocardin-Related Transcription Factors A and B (MRTF-A and MRTF-B) are highly homologous proteins that function as powerful coactivators of serum response factor (SRF), a ubiquitously expressed transcription factor essential for cardiac development. The SRF/MRTF complex binds to CArG boxes found in the control regions of genes that regulate cytoskeletal dynamics and muscle contraction, among other processes. While SRF is required for heart development and function, the role of MRTFs in the developing or adult heart has not been explored. Through cardiac-specific deletion of MRTF alleles in mice, we show that either MRTF-A or MRTF-B is dispensable for cardiac development and function, whereas deletion of both MRTF-A and MRTF-B causes a spectrum of structural and functional cardiac abnormalities. Defects observed in MRTF-A/B null mice ranged from reduced cardiac contractility and adult onset heart failure to neonatal lethality accompanied by sarcomere disarray. RNA-seq analysis on neonatal hearts identified the most altered pathways in MRTF double knockout hearts as being involved in cytoskeletal organization. Together, these findings demonstrate redundant but essential roles of the MRTFs in maintenance of cardiac structure and function and as indispensible links in cardiac cytoskeletal gene regulatory networks.
Overall design P0 Heart mRNA profiles of wild-type (WT) and MRTFA/B double knockout animals were generated in duplicate using DeepSeq using Illumina HiSeq 2500
Contributor(s) Bassel-Duby R, Olson E
Citation(s) 26386146
Submission date Jun 10, 2015
Last update date May 15, 2019
Contact name Eric N Olson
Phone 214-648-1187
Fax 214-648-1196
Organization name University of Texas Southwestern Medical Center at Dallas
Department Molecular Biology
Lab Olson Lab
Street address 6000 Harry Hines Blvd.
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (4)
GSM1707908 Sample_WT1
GSM1707909 Sample_WT2
GSM1707910 Sample_KO1
BioProject PRJNA286287
SRA SRP059315

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69726_RAW.tar 730.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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