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Series GSE69610 Query DataSets for GSE69610
Status Public on Dec 09, 2015
Title Genome wide mapping of H3K27me3 in differentiated mouse podocytes
Organism Mus musculus
Experiment type Other
Summary We explored H3K27me3 binding sites in the genome of differentiated, conditionally immortalized mouse podocytes. Cells were allowed to differentiate for 14 days, following thermoshifting, before treatment with either vehicle (DMSO) or the S-adenosylhomocysteine hydrolase inhibitor 3-deazaneplanocin A (DZNep, 5┬ÁM for 48 hours) which degrades the histone methyltransferase, EZH2 ordinarily responsible for H3K27 trimethylation (H3K27me3). DNA was immunopreciptated with an H3K27me3-specific antibody.
 
Overall design Studying H3K27me3 modification in Mouse Podocyte
 
Contributor(s) Advani A
Citation(s) 26534922
Submission date Jun 05, 2015
Last update date May 15, 2019
Contact name Andrew Advani
E-mail(s) advania@smh.ca
Phone 4168646060
Organization name St. Michael's Hospital
Department Medicine
Street address 6-151 61 Queen Street East
City Toronto
State/province Ontario
ZIP/Postal code M5C 2T2
Country Canada
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (7)
GSM1704711 M_Pod_Control_H3K27me3 Chip-seq_1
GSM1704712 M_Pod_Control_H3K27me3 Chip-seq_2
GSM1704713 M_Pod_Control_H3K27me3 Chip-seq_3
Relations
BioProject PRJNA285980
SRA SRP059190

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69610_RAW.tar 578.5 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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