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Series GSE69483 Query DataSets for GSE69483
Status Public on Jun 03, 2015
Title Control of stomach smooth muscle development and intestinal rotation by transcription factor BARX1 (ChIP-seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Diverse functions of the homeodomain transcription factor BARX1 include Wnt-dependent, non-cell autonomous specification of the stomach epithelium, tracheo-bronchial septation, and Wnt-independent expansion of the spleen primordium. Tight spatio-temporal regulation of Barx1 levels in the mesentery and stomach mesenchyme suggests additional roles. To determine these functions, we forced constitutive BARX1 expression in the Bapx1 expression domain, which includes the mesentery and intestinal mesenchyme, and also examined Barx1-/- embryos in further detail. Transgenic embryos invariably showed intestinal truncation and malrotation, in part reflecting abnormal left-right patterning. Ectopic BARX1 expression did not affect intestinal epithelium, but intestinal smooth muscle developed with features typical of the stomach wall. BARX1, which is normally restricted to the developing stomach, drives robust smooth muscle expansion in this organ by promoting proliferation of myogenic progenitors at the expense of other sub-epithelial cells. Undifferentiated embryonic stomach and intestinal mesenchyme showed modest differences in mRNA expression and BARX1 was sufficient to induce much of the stomach profile in intestinal cells. However, limited binding at cis-regulatory sites implies that BARX1 may act principally through other transcription factors. Genes expressed ectopically in BARX1+ intestinal mesenchyme and reduced in Barx1-/- stomach mesenchyme include Isl1, Pitx1, Six2 and Pitx2, transcription factors known to control left-right patterning and influence smooth muscle development. The sum of evidence suggests that potent BARX1 functions in intestinal rotation and stomach myogenesis occur through this small group of intermediary transcription factors.
Overall design We produced lentivirus to express 3xFLAG-BARX1-P2A-EGFP, infected P53DD-immortalized E13.5 stomach mesenchymal cells, and performed ChIP-seq using FLAG antibody
Contributor(s) Jayewickreme CD, Shivdasani RA
Citation(s) 26057579
Submission date Jun 02, 2015
Last update date May 15, 2019
Contact name Chenura Jayewickreme
Organization name Weill Cornell Medical College
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM1701912 Flag-Barx1_ChIP
GSM1701913 Flag-Barx1_Input
BioProject PRJNA285703
SRA SRP059026

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Supplementary file Size Download File type/resource
GSE69483_RAW.tar 862.5 Mb (http)(custom) TAR (of BED, BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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