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Series GSE69445 Query DataSets for GSE69445
Status Public on Jun 15, 2016
Title Crohn's disease risk variant in GPR65 alters lysosomal function
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Using an siRNA screen we identify a role for GPR65 in the defense against intracellular pathogens. Epithelial cells and macrophages lacking GPR65 exhibited impaired clearance of intracellular bacteria as well as an accumulation of aberrant phagosomes and lysosomes. Transcriptional profiling revealed changes in genes associated with lysosomal function.
Overall design Bone marrow-derived macrophages from WT or Gpr65-/- mice were harvested for RNA analysis.
Contributor(s) Lassen K, Goel G, Xavier RJ
Citation(s) 27287411
Submission date Jun 01, 2015
Last update date May 15, 2019
Contact name Ramnik J Xavier
Organization name Massachusetts General Hospital
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM1701317 WT_BMDMs_T0_1
GSM1701318 WT_BMDMs_T0_2
GSM1701319 WT_BMDMs_T0_3
BioProject PRJNA285503
SRA SRP058951

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69445_KL.GPR65.raw.UMI.gct.txt.gz 138.0 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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