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Series GSE69351 Query DataSets for GSE69351
Status Public on Jul 16, 2015
Title Integrative analyses of human reprogramming reveal dynamic nature of induced pluripotency [SCRB-Seq]
Organisms Homo sapiens; Mus musculus; synthetic construct
Experiment type Expression profiling by high throughput sequencing
Summary Induced pluripotency is a promising avenue for disease modeling and therapy, but the molecular principles underlying this process, particularly in human cells, remain poorly understood due to donor-to-donor variability and intercellular heterogeneity. Here we constructed and characterized a clonal, inducible human reprogramming system that provides a reliable source of cells at any stage of the process. This system enabled integrative transcriptional and epigenomic analysis across the human reprogramming timeline at high resolution. We observed distinct waves of gene network activation, including the ordered re-activation of broad developmental regulators followed by early embryonic patterning genes and culminating in the emergence of a signature reminiscent of pre-implantation stages. Moreover, complementary functional analyses allowed us to identify and validate novel regulators of the reprogramming process. Altogether, this study sheds light on the molecular underpinnings of induced pluripotency in human cells and provides a robust cell platform for further studies.
Overall design hiF-T cells are secondary reprogrammable cells derived from hiF cells obtained thorough the directed differentiation of a dox-inducible OKMS hIPSC line obtained from reprogramming BJ fibroblasts. After directed differentiation, hiF cells were infected with a lentivirus expressing the human telomerase and after clonal expansion individual hiF-T clones were obtained.

The multiplexed data is available at:
Contributor(s) Cacchiarelli D, Soumillon M, Ziller MJ, Ho Sui SJ
Citation(s) 26186193
Submission date May 29, 2015
Last update date May 15, 2019
Contact name Shannan J Ho Sui
Organization name Harvard T.H. han Scholol of Public Health
Department Biostatistics
Street address 655 Huntington Ave, Building 2, Rm 437A
City Boston
State/province Massachusetts
ZIP/Postal code 02115
Country USA
Platforms (3)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19604 Illumina HiSeq 2500 (synthetic construct)
Samples (96)
GSM1698374 P3_A1
GSM1698375 P3_A2
GSM1698376 P3_A3
This SubSeries is part of SuperSeries:
GSE62777 Integrative analyses of human reprogramming reveal dynamic nature of induced pluripotency
BioProject PRJNA285283
SRA SRP058840

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69351_all_genes_refseq_3DGE.UMI_counts.txt.gz 1.5 Mb (ftp)(http) TXT
GSE69351_barcodes_trugrade_96_set1.txt.gz 590 b (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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