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Series GSE69206 Query DataSets for GSE69206
Status Public on Mar 21, 2016
Title Comparing effects of perfusion and hydrostatic pressure on human chondrocytes using gene profiles
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Hydrostatic pressure and perfusion have been shown to alter the chondrogenic potential of articular chondrocytes. In order to compare the effects of hydrostatic pressure plus perfusion (HPP) and perfusion (P) we investigated the complete gene expression profiles of human chondrocytes under HPP and P. A simplified bioreactor was constructed applying loading (0.1 MPa for 2 h) and perfusion (2ml) through the same piping by pressurizing the medium directly. High-density monolayer cultures of human chondrocytes were exposed to HPP or P for 4 days. Controls were maintained in static culture. Gene expression was evaluated by sequencing (RNAseq) and quantitative real-time PCR analysis. RNAseq identified similarities between the two treatments. Specifically, HPP and P increased COL2A1 expression and decreased COL1A1 and MMP-13 expression. Despite of the similarities, RNAseq revealed a list of cartilage genes including ACAN, ITGA10 and TNC, which were differentially expressed by HPP and P. Of these candidates adhesion related molecules were found to be upregulated in HPP. Both HPP and P treatment had beneficial effects on chondrocyte differentiation and decreased catabolic enzyme expression. The study provides new insight into how hydrostatic pressure and perfusion enhance cartilage differentiation and inhibit catabolic effects
Overall design 9 samples
Contributor(s) Zhu G, Meyer-Wagner S, Schröder C, Woiczinski M, Blum H, Lavagi I, Krebs S, Redeker JI, Hölzer A, Jansson V, Betz O, Müller PE
Citation(s) 26133928
Submission date May 26, 2015
Last update date May 15, 2019
Contact name Stefan Krebs
Phone 0049-89-2180 76715
Organization name Ludwig-Maximilian University
Department Gene Center
Lab Lafuga Genomics
Street address Feodor-Lynen-Str. 25
City Munich
ZIP/Postal code 81377
Country Germany
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (9)
GSM1695254 control replicate1
GSM1695255 control replicate2
GSM1695256 control replicate3
BioProject PRJNA284885
SRA SRP058698

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Supplementary file Size Download File type/resource
GSE69206_counts.txt.gz 278.5 Kb (ftp)(http) TXT
GSE69206_rlog.txt.gz 1015.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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