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| Status |
Public on May 31, 2016 |
| Title |
TNFalpha and IL-6 induced anorexia: effects on serotonin turnover |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Anorexia can occur as a serious complication of chronic disease. Increasing evidence suggests that inflammation plays a major role, along with a hypothalamic dysregulation characterized by locally elevated serotonin levels. The present study was undertaken to further explore the connections between peripheral inflammation, anorexia and hypothalamic serotonin metabolism and signaling pathways. We studied transcriptomic changes and serotonergic activity in the hypothalamus of mice after an intraperitoneal injection with TNFα, IL-6 or a combination of TNFα and IL-6.
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| Overall design |
C57BL/6 mice were intraperitoneally injected with TNFalpha, IL-6 Low, IL-6 High, combination TNF+IL-6 Low or combination TNF+IL-6 High. Each group comprised of 12 animals, of which 6 animals were used for transcriptomics.
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| Contributor(s) |
Dwarkasing J, Witkamp R, Boekschoten MV, ter Laak M, Flik G, van Norren K |
| Citation(s) |
27207102 |
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| Submission date |
May 21, 2015 |
| Last update date |
Apr 18, 2017 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
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| Organization name |
Wageningen University
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| Department |
Div. Human Nutrition & Health
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| Lab |
Nutrition, Metabolism & Genomics Group
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| Street address |
HELIX, Stippeneng 4
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| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (36)
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| GSM1693963 |
hypothalamus, IL6 high, replicate 1 |
| GSM1693964 |
hypothalamus, combination 1 (TNF+IL6 low), replicate 1 |
| GSM1693965 |
hypothalamus, combination 2 (TNF+IL6 high), replicate 1 |
| GSM1693966 |
hypothalamus, control, replicate 2 |
| GSM1693967 |
hypothalamus, TNFa low, replicate 2 |
| GSM1693968 |
hypothalamus, IL6 low, replicate 2 |
| GSM1693969 |
hypothalamus, IL6 high, replicate 2 |
| GSM1693970 |
hypothalamus, combination 1 (TNF+IL6 low), replicate 2 |
| GSM1693971 |
hypothalamus, combination 2 (TNF+IL6 high), replicate 2 |
| GSM1693972 |
hypothalamus, control, replicate 3 |
| GSM1693973 |
hypothalamus, TNFa low, replicate 3 |
| GSM1693974 |
hypothalamus, IL6 low, replicate 3 |
| GSM1693975 |
hypothalamus, IL6 high, replicate 3 |
| GSM1693976 |
hypothalamus, combination 1 (TNF+IL6 low), replicate 3 |
| GSM1693977 |
hypothalamus, combination 2 (TNF+IL6 high), replicate 3 |
| GSM1693978 |
hypothalamus, control, replicate 4 |
| GSM1693979 |
hypothalamus, TNFa low, replicate 4 |
| GSM1693980 |
hypothalamus, IL6 low, replicate 4 |
| GSM1693981 |
hypothalamus, IL6 high, replicate 4 |
| GSM1693982 |
hypothalamus, combination 1 (TNF+IL6 low), replicate 4 |
| GSM1693983 |
hypothalamus, combination 2 (TNF+IL6 high), replicate 4 |
| GSM1693984 |
hypothalamus, control, replicate 5 |
| GSM1693985 |
hypothalamus, TNFa low, replicate 5 |
| GSM1693986 |
hypothalamus, IL6 low, replicate 5 |
| GSM1693987 |
hypothalamus, IL6 high, replicate 5 |
| GSM1693988 |
hypothalamus, combination 1 (TNF+IL6 low), replicate 5 |
| GSM1693989 |
hypothalamus, combination 2 (TNF+IL6 high), replicate 5 |
| GSM1693990 |
hypothalamus, control, replicate 6 |
| GSM1693991 |
hypothalamus, TNFa low, replicate 6 |
| GSM1693992 |
hypothalamus, IL6 low, replicate 6 |
| GSM1693993 |
hypothalamus, IL6 high, replicate 6 |
| GSM1693994 |
hypothalamus, combination 1 (TNF+IL6 low), replicate 6 |
| GSM1693995 |
hypothalamus, combination 2 (TNF+IL6 high), replicate 6 |
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| Relations |
| BioProject |
PRJNA284645 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE69151_RAW.tar |
161.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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