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Series GSE68974 Query DataSets for GSE68974
Status Public on Jul 23, 2015
Title Genome-wide chromatin-immunoprecipitation studies of purified SAN cells reveals critical direct targets of ISL1 and suggests potential co-factor families for ISL1 action in SAN cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Understanding factors that drive development and function of the sinoatrial node (SAN) is crucial to development of potential therapies for sinus arrhythmias, including potential generation of biological pacemakers. Here, we identify a key cell autonomous role for the LIM homeodomain transcription factor ISL1 for survival, proliferation and function of pacemaker cells throughout development. Chromatin immunoprecipitation assays performed utilizing antibody to ISL1 in chromatin extracts from FACS purified SAN cells demonstrated that ISL1 directly binds genomic regions within several genes critical for normal pacemaker function, including subunits of the L-type calcium channel, Ank2, and Tbx3. Other genes implicated in abnormal heart rhythm in humans were also direct downstream targets of ISL1 in SAN cells. Our studies represent the first in vivo ChIP-seq studies for SAN cells which provide a basis for further exploration of factors critical to SAN formation and function and highlight the potential for utilization of ISL1 in combination with other SAN transcription factors for generating pacemaker cells for therapy or drug screening purposes.
Overall design ISL1 ChIP-seq profiling was performed in Hcn4-H2BGFP SAN cells purified from neonatal hearts.
Contributor(s) Cattaneo P, Spann NJ, Liang X, Zhang Q, Gong X, Zhuang S, Glass CK, Chen J, Sun Y, Evans SM
Citation(s) 26193633
Submission date May 18, 2015
Last update date May 15, 2019
Contact name Paola Cattaneo
Organization name National Research Council of Italy
Department Institute of Genetic and Biomedical Research
Street address Via Fantoli 16/15
City Milan
ZIP/Postal code 20138
Country Italy
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (2)
GSM1689148 SAN_ChIP-seq_ISL1
GSM1689149 SAN_ChIP-seq_input DNA
BioProject PRJNA284245
SRA SRP058432

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Supplementary file Size Download File type/resource
GSE68974_RAW.tar 120.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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