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Series GSE68789 Query DataSets for GSE68789
Status Public on Aug 31, 2015
Title Distinct murine mucosal Langerhans cell subsets develop from pre-DCs and monocytes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Langerhans cells (LCs) populate the mucosal epithelium, a major entry portal for pathogens, yet their ontogeny remains unclear. In contrast to skin LCs originating from self-renewing radioresistant embryonic precursors, we found that oral mucosal LCs derive from circulating radiosensitive precursors. Mucosal LCs can be segregated into CD103+CD11blow (CD103+LCs) and CD11b+CD103- (CD11b+LCs) subsets. We further demonstrated that similar to non-lymphoid dendritic cells (DCs), CD103+LCs originate from pre-DCs, whereas CD11b+LCs differentiate from both pre-DCs and monocytic precursors. Despite this ontogenetic discrepancy between skin and mucosal LCs, transcriptomic signature and immunological function of oral LCs highly resemble those of skin LCs but not DCs. These findings, along with their epithelial position, morphology and expression of LC-associated phenotype strongly suggest that oral mucosal LCs are genuine LCs. Collectively, in a tissue-dependent manner, murine LCs differentiate from at least three distinct precursors (embryonic, pre-DCs and monocytic) in steady state
Overall design The following cells were isolated from mice (2-4 replicates): Lung DCs, mucosal CD103+ LC, mucosal CD11b+ LC, Skin LC. Transcriptome analysis was performed.
Citation(s) 26231115
Submission date May 12, 2015
Last update date May 15, 2019
Contact name Ido Amit
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (14)
GSM1681434 Lungs_Langerin_P_CD103_P_Rep1
GSM1681435 Lungs_Langerin_P_CD103_P_Rep2
GSM1681436 Mucosal_CD103_P_LC_Rep1
SRA SRP058229
BioProject PRJNA283850

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Supplementary file Size Download File type/resource
GSE68789_20150128_Ronnie.n_counts.txt.gz 320.9 Kb (ftp)(http) TXT
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