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Series GSE6872 Query DataSets for GSE6872
Status Public on Apr 27, 2007
Title Spermatozoal RNA Profiles (U133 Plus 2.0 Array)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Normal human spermatogenesis concludes with the formation of large numbers of morphologically well developed spermatozoa. While transcriptionally quiescent these cells carry an RNA payload that reflects the final spermiogenic phase of transcription. We report here the spermatozoal transcript profiles characteristic of normally fertile individuals and infertile males suffering from a consistent and severe teratozoospermia in which under 4% of spermatozoa are morphologically normal. RNA was extracted from the purified sperm cells of ejaculate and hybridized to Affymetrix U133 (v2) Microarrays.
Keywords: disease state analysis
 
Overall design Spermatozoal RNAs were prepared from the semen samples of 21 individuals. An asymmetric dual block design was adopted with biological replicates in both blocks. 13 semen samples were assessed from normally fertile males who had fathered at least one child. 8 semen samples were assessed from infertile individuals with a severe and consistent heterogeneous teratozoospermia who showed no other abnormal semen parameters.
Web link http://compbio.med.wayne.edu
 
Contributor(s) Platts AE, Dix DJ, Chemes HE, Thompson KE, Goodrich R, Rockett JC, Rawe VY, Quintana S, Diamond MP, Strader LF, Krawetz SA
Citation(s) 17327269
Submission date Jan 25, 2007
Last update date Mar 25, 2019
Contact name Stephen A Krawetz
E-mail(s) steve@compbio.med.wayne.edu
Organization name Wayne State University
Street address 275 East Hancock, CS Mott Center
City Detroit
State/province MI
ZIP/Postal code 48207
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (21)
GSM158463 Normospermic individual: Sample N1
GSM158464 Normospermic individual: Sample N10
GSM158465 Normospermic individual: Sample N11
This SubSeries is part of SuperSeries:
GSE6969 Spermatozoal RNA Profiles from Normally Fertile and Heterogeneously Teratozoospermic Subjects
Relations
BioProject PRJNA104107

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6872_RAW.tar 138.4 Mb (http)(custom) TAR (of CEL)

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