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Series GSE68682 Query DataSets for GSE68682
Status Public on Dec 08, 2016
Title Cell density, Her2 and progesterone signaling regulate dissemination of breast cancer cells [miRNA-Seq]
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Disseminated breast cancer cells display genotypes disparate from the predominant clone of the primary tumor before manifestation of metastasis, suggesting that cancer cell dissemination occurs preferentially early; however, the underlying molecular mechanisms are unknown. Investigating metastasis in a Her2-driven mouse model, we found that the progesterone-induced paracrine cytokines Wnt4 and Rankl induced migration and early dissemination shortly after Her2 activation. Once tumorigenic growth was established, progesterone receptor (PgR) expression was lost and Wnt4/Rankl induced proliferation. The altered response from migration to proliferation was determined by cell density involving miRNA-regulated PgR expression and was reversible. Cells from early, low-density lesions displayed more functional stemness traits than cells from dense, advanced tumors, migrated more and founded significantly more metastases. The data suggest that many metastases are derived from early-disseminated cancer cells, implying that our concepts for systemic therapy need to be revised.
Overall design We analysed miRNA expression in the Her2 expressing cell lines as well as the secreting miRNA containing particles to find out PgR regulating miRNAs
Contributor(s) Hosseini H, Hoffmann M, Meister G, Klein CA
Citation(s) 27974799
Submission date May 08, 2015
Last update date May 15, 2019
Contact name Christoph Andreas Klein
Organization name University of Regensburg
Department Experimental Medicine and Therapy Research
Street address Franz-Josef-Strauß-Allee 11
City Regensburg
State/province Bavaria
ZIP/Postal code 93053
Country Germany
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (15)
GSM1678987 Exosomes, replicate #1
GSM1678988 Exosomes, replicate #2
GSM1678989 Exosomes, replicate #3
This SubSeries is part of SuperSeries:
GSE68683 Cell density, Her2 and progesterone signaling regulate dissemination of breast cancer cells
BioProject PRJNA283389
SRA SRP058132

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68682_DeepSeq-Results_Summary_Hosseini_Klein.xlsx 271.2 Kb (ftp)(http) XLSX
GSE68682_RAW.tar 4.9 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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