GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE68378 Query DataSets for GSE68378
Status Public on Jan 26, 2016
Title Ambient O2 pressure induces NF-kB1/RelA related inflammatory response in human lung epithelial cells in vitro
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Oxygen (O2) levels in cell culture conditions is typically 2-5 fold higher than the physiological O2 levels that most tissues experience in vivo. The ambient atmospheric O2 (21%) is known to induce cell proliferation defects and cellular senescence in stem cell and primary cell cultures. Therefore, culturing these cells under lower O2 levels (2-9%) is currently a standard practice. However, the non-cancerous immortalized cells and cancer cells, which evade cellular senescence are normally cultured under 21% O2 levels and the effects of higher O2 levels on these cells are not fully understood.
Methods: Gene expression (RNA seq transcriptomics) analysis of immortalized human bronchial epithelial (BEAS-2B) cells cultured at ambient 21% O2 and lower 10% O2 levels for 3 days and 3 weeks. Further the beneficial effects of cuturing cells under lower oxygen tension is evalulated
Results: Our results show NF-κB/RelA mediated activation of pro-inflammatory cytokines as a major outcome of cells being cultured 21% O2. Moreover, we demonstrate increased RelA binding at the NF-κB1/RelA target gene promoters at 21% O2. Interestingly, contrary to cells cultutred at 21% O2, external stress induced by H2O2 exposure did not induce inflammatory response in cells grown at 10% O2, suggesting increased ability to handle external stress in cells cultured at lower O2 levels.
Overall design RNA Seq gene expression comparision done in replicates
Contributor(s) Cuddapah S, Coasta M, Jagannathan L
Citation(s) 26588041
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 ES023174 Epigenetic Dysregulation by Oxidative Stress from Environmental Insults NEW YORK UNIVERSITY SCHOOL OF MEDICINE Cuddapah
R01 ES024727 Epigenetic reprogramming by nickel through chromatin domain disruption NEW YORK UNIVERSITY SCHOOL OF MEDICINE Cuddapah
Submission date Apr 28, 2015
Last update date May 15, 2019
Contact name Suresh Cuddapah
Organization name New York University Langone Medical center
Department Environmental Sciecne
Street address 57 Old Forge Road
City Tuxedo
State/province New York
ZIP/Postal code 10987
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (10)
GSM1669552 control-10-3d-Replicate 1
GSM1669553 control-10-3d-Replicate 2
GSM1669554 control-21-Replicate 1
BioProject PRJNA282543
SRA SRP057766

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68378_RAW.tar 6.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap