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Series GSE68376 Query DataSets for GSE68376
Status Public on Jul 15, 2015
Title Interleukin-1 signaling is involved in local self-renewal and maintenance of microglia
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary During early embryogenesis microglia arise from yolk sac progenitors populating the developing CNS, where they are maintained as tissue-resident macrophages throughout the organism’s lifespan.
Here, we describe an experimental system that allows the specific conditional ablation of microglia in vivo.
Strikingly, we found that the microglia compartment was reconstituted within one week following depletion. Microglia repopulation relied entirely on a CNS-resident, internal pool and was independent from bone marrow-derived precursors.
Newly formed microglia were found in highly proliferative, organized micro-clusters that dissolve once steady state was achieved. Gene expression profiling revealed prominent expression of Interleukin-1 (IL-1) receptor in proliferating microglia.
During the repopulation phase, IL-1 signaling was neutralized by treatment with IL-1 receptor antagonist that impaired microglia proliferation. Hence, microglia harbor a highly efficient potential to restore themselves without contribution of peripheral myeloid cells.
IL-1 signaling significantly participates in this restorative proliferation process and is involved in stabilizing microglia maintenance.
Overall design bone marrow macrophages, wild type microglia, and repopulating microglia
Contributor(s) Waisman A, Bruttger J, Marini F
Citation(s) 26163371
Submission date Apr 28, 2015
Last update date May 15, 2019
Contact name Ari Waisman
Organization name Institute for Molecular Medicine
Street address Obere Zahlbacher Straße 67
City Mainz
ZIP/Postal code 55131
Country Germany
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (8)
GSM1669529 bm_micro_rep1
GSM1669530 bm_micro_rep2
GSM1669531 bm_micro_rep3
BioProject PRJNA282538
SRA SRP057763

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Supplementary file Size Download File type/resource
GSE68376_normalizedCounts.txt.gz 738.0 Kb (ftp)(http) TXT
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