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Series GSE68221 Query DataSets for GSE68221
Status Public on Jul 01, 2015
Title Histone variant H2A.Z.2 mediates proliferation and drug sensitivity of malignant melanoma [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Here we report a novel role for H2A.Z.2 (H2AFV) as a mediator of cell proliferation and sensitivity to targeted therapies in malignant melanoma. While both H2A.Z.1 and H2A.Z.2 are highly expressed in metastatic melanoma and correlate with decreased patient survival, only H2A.Z.2 deficiency results in impaired cellular proliferation of melanoma cells, which occurs via a G1/S arrest. Integrated gene expression and ChIP-seq analyses revealed that H2A.Z.2 positively regulates E2F target genes, and that such genes acquire a distinct H2A.Z occupancy signature over the promoter and gene body in metastatic melanoma cells. We further identified the BET family member BRD2 as an H2A.Z-interacting protein in melanoma cells, and demonstrate that H2A.Z.2 silencing cooperates with BET inhibition to induce cell death.
 
Overall design Expression levels for non tumorigenic (Melanocytes) and human melanoma cell line SKmel147, before and after JQ1 treatement
 
Contributor(s) Vardabasso C, Gaspar-Maia A, Hasson D, Pu╠łnzeler S, Valle-Garcia D, Straub T, Keilhauer EC, Strub T, Dong J, Panda T, Chung C, Yao JL, Singh R, Segura MF, Fontanals-Cirera B, Verma A, Mann M, Hernando E, Hake SB, Bernstein E
Citation(s) 26051178
Submission date Apr 23, 2015
Last update date May 15, 2019
Contact name Emily Bernstein
E-mail(s) emily.bernstein@mssm.edu
Phone (212) 824-9335
Organization name Mount Sinai School of Medicine
Department Oncological Sciences
Lab Bernstein Lab
Street address 1470 Madison Avenue, 6th floor Rm 302
City New York
State/province NY
ZIP/Postal code 10029
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (3)
GSM1665987 Mel-RNA
GSM1665988 SKmel147-control-RNA
GSM1665989 SKmel147-JQ1-RNA
This SubSeries is part of SuperSeries:
GSE59060 Histone variant H2A.Z.2 mediates proliferation and drug sensitivity of malignant melanoma
Relations
BioProject PRJNA282049
SRA SRP057616

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68221_RAW.tar 407.3 Mb (http)(custom) TAR (of BIGWIG, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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