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Series GSE68139 Query DataSets for GSE68139
Status Public on May 01, 2016
Title Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary How the parental genomes of the very specialized sperm and oocyte cells are remodelled upon fertilization to confer totipotency has remained a tantalizing open questions. Indeed, in the case of mammals, the parental genomes undergo dramatic reprogramming upon fertilization, including differential dynamics of histone post-translational modifications. The roles of histone modifying enzymes in this process, which are maternally provided, are only just starting to emerge. Here, we explore the function of the oocyte inherited pool of Lsd1/Kdm1a, which encodes a histone H3K4 and K9 demethylase, during early mouse development. Maternal deficiency of Lsd1/Kdm1a results in developmental arrest by the two-cell stage, associated with dramatic and stepwise alterations in H3K9 and H3K4 methylation patterns depending on its demethylase activity. At the transcriptional level, two major changes occur. On one hand, switch from maternal-to-zygotic program fails to be induced. On the other hand, LINE-1 retrotransposons are not properly silenced, along with evidences for increased LINE-1 activity. We propose that Lsd1/Kdm1a is involved in the correct establishment of epigenetic information harboured by histones and is involved in the initiation of new pattern of genome expression driving early mouse development and preserving genome integrity
Overall design RNA-seq of invidual mouse two-cell stage embryos
Contributor(s) Ancelin K, Syx L, Servant N, Chen C, Heard E
Citation(s) 26836306
Submission date Apr 21, 2015
Last update date May 15, 2019
Contact name Nicolas Servant
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (16)
GSM1664485 f/wt two cell embryo 1 RNA seq
GSM1664486 f/wt two cell embryo 2 RNA seq
GSM1664487 f/wt two cell embryo 3 RNA seq
SRA SRP057536
BioProject PRJNA281886

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Supplementary file Size Download File type/resource
GSE68139_RAW.tar 11.2 Mb (http)(custom) TAR (of XLS)
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Raw data are available in SRA
Processed data provided as supplementary file

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