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Series GSE68069 Query DataSets for GSE68069
Status Public on Apr 11, 2016
Title Master transcription factors in corneal epithelial cells [ATAC-Seq]
Organism Homo sapiens
Experiment type Other
Genome binding/occupancy profiling by high throughput sequencing
Summary In development, embryonic ectoderm differentiates into several lineages including neuroectoderm and surface ectoderm, through the mechanism largely unclear. Here we report that OVOL2 is required for the transcriptional program of corneal epithelium cell(CEC)s, a derivative of surface ectoderm, and it might regulates the differential transcriptional programs between the two lineages. By a functional screening, we identified transcription factors (TFs) maintaining human CECs. OVOL2 was necessary to maintain the transcriptional program in CECs, particularly through repressing expression of mesenchymal genes. OVOL2 combined with several TFs were able to activate the transcriptional program of CECs in fibroblasts, accompanied by induction of chromatin landscape. Moreover, our analysis revealed that neuroectoderm derivatives express some of mesenchymal genes. In fact, OVOL2 alone was able to induce the transcriptional program of CECs in neural progenitor cells (NPCs) through repression of mesenchymal genes as well as activation of epithelial genes. Our data suggest that the difference between the transcriptional programs of surface ectoderm-derivatives and neuroectoderm-derivatives is regulated in part by the reciprocally-repressive mechanism between epithelial and mesenchymal genes that is seen in epithelial-to-mesenchymal transition.
Overall design ATAC-Seq protocol performed on: Human primary CECs; N=4, 6TFs transduced fibroblasts; N=4, fibroblasts; N=4
Contributor(s) Kitazawa K, Kinoshita S, Masui S
Citation(s) 27134177
Submission date Apr 20, 2015
Last update date Jun 10, 2019
Contact name Koji Kitazawa
Organization name Kyoto Prefectural University of Medicine
Department Ophthalmology
Street address 465 Kajiicho Kamigyoku
City Kyoto
State/province Kyoto
ZIP/Postal code 601-0841
Country Japan
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (12)
GSM1662250 ATAC-Seq_Sample01_6TFs_Rep1
GSM1662251 ATAC-Seq_Sample01_6TFs_Rep2
GSM1662252 ATAC-Seq_Sample01_6TFs_Rep3
This SubSeries is part of SuperSeries:
GSE67823 Master transcription factors in corneal epithelial cells
BioProject PRJNA281650
SRA SRP057473

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68069_RAW.tar 14.1 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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