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Series GSE68031 Query DataSets for GSE68031
Status Public on Sep 20, 2020
Title miRNA transcriptome profiling of spheroid-enriched cells with cancer stem cell properties in human breast MCF-7 cell line.
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Intro: Spheroid culture especially on MCF-7 cell has been used as in vitro CSC model for anti-CSC drug discovery.
Methods: Tumour spheroid of MCF-7 was generated and characterised for the biological features and CSCs characteristic comparing to the monolayer parental MCF-7 cells. Differential expression of miRNA between the spheroid and parental cells was evaluated using miRNA microarray expressions.
 
Overall design miRNA expression profiles of MCF-7 enriched CSCs spheroids and monolayer cultures were generated in triplicates by Affymetrix GeneChip miRNA_4.0 array.
 
Contributor(s) Boo L, Ho WY, Ali NM, Yeap SK, Ky H, Chan KG, Yin WF, Satharasinghe DA, Liew WC, Tan SW, Ong HK, Cheong SK
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Submission date Apr 20, 2015
Last update date Sep 22, 2020
Contact name norlaily mohd ali
E-mail(s) norlailyma@gmail.com
Organization name Universiti Tunku Abdul Rahman
Department Faculty of Medicine and Health Sciences
Street address Universiti Tunku Abdul Rahman, Jln Sg Long
City Cheras
State/province Selangor
ZIP/Postal code 43000
Country Malaysia
 
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (6)
GSM1661397 MCF7_P_1
GSM1661398 MCF7_P_2
GSM1661399 MCF7_P_3
Relations
BioProject PRJNA281587

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68031_RAW.tar 4.9 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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