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Series GSE67945 Query DataSets for GSE67945
Status Public on May 08, 2015
Title Investigation about fibroblasts of different origins in culture
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this study was to determine if fibroblasts from different origin (skin, colon, tumors) were keeping their characteristic while extracted and cultured ex vivo for several passages. HUVEC was used as a control, being cells from a different background. Surprisingly, fibroblasts from different origins are losing their independant characteristic to cluster in a similar way after 5-6 passages in culture in vitro, showing an activated status.
Overall design Fibroblasts were extracted from human skin, colon normal stroma and colon tumor stroma. HUVECs were extracted from human samples at the same time. All cells, each group from 3 different patients, were grown on plastic for 5 passages and mRNA was extracted to perform RNASeq analysis.
Contributor(s) Rüegg C, Knuchel S, Anderle P
Citation(s) 25973543
Submission date Apr 16, 2015
Last update date May 15, 2019
Contact name Rüegg Curzio
Organization name Université de Fribourg
Department Département de Médecine 3
Lab Pathology
Street address Albert-Gockel 1
City Fribourg
ZIP/Postal code 1700
Country Switzerland
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (12)
GSM1659543 cFB_N_01
GSM1659544 cFB_N_02
GSM1659545 cFB_N_03
BioProject PRJNA281358
SRA SRP057251

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67945_RAW.tar 2.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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