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Series GSE67866 Query DataSets for GSE67866
Status Public on May 28, 2015
Title RNA sequencing of cells treated with DMSO or Retinoic acid during cardiac differentiation
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Analysis of transcriptional differences between control and RA-treated cells during cardiac differentiation. The hypothesis tested in these samples is that addition of RA during differentiation towards atrial-like cardiomyocytes while control cells treated with DMSO result in ventricular-like cardiomyocytes.
Overall design NKX2.5 (eGFP/w)-hESCs were differentiated to cardiomyocytes with spin EB protocol, with the addition of RA or DMSO. Cells were sorted at day-31 based on GFP resulting in CTplus, CTminus, RAplus or RAminus goups. RNA was isolated from each of these fractions for sequencing.
Contributor(s) Roost MS, van Iperen L, Ariyurek Y, Buermans HP, Arindrarto W, Devalla HD, Passier R, Mummery CL, Carlotti F, de Koning EJ, van Zwet EW, Goeman JJ, de Sousa Lopes SM
Citation(s) 26028532
Submission date Apr 14, 2015
Last update date May 15, 2019
Contact name Nannan He
Organization name Leiden University Medical Center
Street address Einthovenweg 20
City Leiden
ZIP/Postal code 2333 ZC
Country Netherlands
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (4)
GSM1657360 NKX2.5:GFP+ RA
GSM1657361 NKX2.5:GFP- RA
GSM1657362 NKX2.5:GFP+ CTR
BioProject PRJNA281093
SRA SRP057156

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Supplementary file Size Download File type/resource
GSE67866_Roost_et_al_Raw_Reads.txt.gz 355.0 Kb (ftp)(http) TXT
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Raw data are available in SRA

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