|
Status |
Public on Dec 07, 2015 |
Title |
Genome-wide maps of BAF180 and REV-ERBα binding |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
We generated genome-wide cistromes of BAF180 subunit of the SWI-SNF chromatin remodeling complex in mouse liver at CT10 and CT22. In addition, we performed ChIP-Seq analysis on REV-ERBα in WT and SRC-2-/- mouse liver at CT10. We found circadian oscilation of BAF180 chromatin recruitment in mouse liver with peak recruitment at CT22 and nadir at CT10. Further,REV-ERBα chromatin recruitment was significantly reduced in SRC-2-/- mouse liver compared to WT mice at CT10.
|
|
|
Overall design |
ChIP-Seq for BAF180 was performed in WT mice liver at CT10 and CT22 using two different antibodies. ChIP-Seq for REV-ERBα was performed in WT and SRC-2-/- mice in liver at CT10 with biological replicates.
|
|
|
Contributor(s) |
Zhu B, York B, O'Malley BW |
Citation(s) |
26611104, 29615789 |
|
Submission date |
Apr 14, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Bokai Zhu |
E-mail(s) |
bzhu@bcm.edu
|
Phone |
2192085319
|
Organization name |
Baylor College of Medicine
|
Department |
MCB
|
Lab |
Post-doc
|
Street address |
One Baylor Plaza, M813
|
City |
Houston |
State/province |
Texas |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platforms (2) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
Samples (7)
|
|
This SubSeries is part of SuperSeries: |
GSE67860 |
Coactivator-Dependent Oscillation of Chromatin Accessibility Dictates Circadian Gene Amplitude through REV-ERB Loading |
|
Relations |
BioProject |
PRJNA281076 |
SRA |
SRP057150 |