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Series GSE67828 Query DataSets for GSE67828
Status Public on Feb 19, 2016
Title Profiling of soma and neurite transcriptomes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report mRNA profiles of subcellularly localized transcriptomes (soma and neurite) of two mouse cell lines, N2A and CAD, as well as primary cortical neurons from E18.5 mice. We also performed this fractionation and sequencing after RNAi knockdown (cell lines) or in knockout mice (primary cortical neurons) of the RNA-binding proteins muscleblind 1 and 2 (Mbnl1 and Mbnl2).
Overall design Fractionate neurons using porous transwell membranes. Isolate poly-A RNA.
Contributor(s) Taliaferro M, Burge C
Citation(s) 26907613
Submission date Apr 13, 2015
Last update date May 15, 2019
Contact name Matthew Taliaferro
Organization name University of Colorado Anschutz Medical Campus
Department Biology
Street address 12801 E 17th Ave, RC1 South Room 10403D
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (48)
GSM1656708 N2ASoma1
GSM1656709 N2ASoma2
GSM1656710 N2ASoma3
BioProject PRJNA281008
SRA SRP057123

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67828_RAW.tar 71.0 Mb (http)(custom) TAR (of FPKM_TRACKING, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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