GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE67809 Query DataSets for GSE67809
Status Public on Apr 14, 2015
Title Genome-wide chromatin binding of transcription factor E2F1 in castration resistant prostate cancer (CRPC) cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Our computational approach identified E2F1 as a collaborative factor for EZH2 in transcriptional regulation of cancer-related genes. This experiment is designed to validate the interaction between E2F1 and EZH2 on the chromatin. By obtaining over 1 billion bases of sequence from chromatin immunoprecipitated DNA, we generated the genome-wide localizations of E2F1 in CRPC cell line LNCaP-abl cells, and found that Indeed, these sites are enriched near the transcription start sites of EZH2-activated genes. Further analysis of the transcription factor motifs enriched at these peaks revealed the enrichement of androgen receptor motif, suggesting a co-activator role for EZH2 in concert with AR. Our work demonstrated a novel funtion of EZH2 in transcriptional activation by directly binding to the chromatin sites that cooperate with AR.
Overall design Study of the chromatin localizations of PRC2 complex core subunits and different histone marks in 2 cell types
Contributor(s) Xu K, Xu H, Chen C
Citation(s) 26659825
Submission date Apr 13, 2015
Last update date May 15, 2019
Contact name Kexin Xu
Organization name UT Health Science Center at San Antonio
Department Molecular Medicine
Street address 7703 Floyd Curl, MC 8257
City San Antonio
State/province TX
ZIP/Postal code 78229
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (2)
GSM1656410 E2F1_ChIPSeq_abl
GSM1656411 input_ChIPSeq_abl
BioProject PRJNA280968
SRA SRP057110

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67809_RAW.tar 468.6 Mb (http)(custom) TAR (of BED, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap