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Series GSE67762 Query DataSets for GSE67762
Status Public on Apr 11, 2015
Title Tissue-engineered human psoriatic skin as an in vitro model to study the impact of cytokines
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Psoriasis is a chronic inflammatory disease of the skin for which no cure has emerged. Its complex etiology requires the development of an in vitro model that appropriately recapitulates the physiopathology of this disease. In this study, we exploited the self-assembly method in order to develop a new tissue-engineered model of psoriatic skin substitutes. To circumvent the addition of immune cells, we supplemented the reconstructed psoriatic substitutes with a cocktail of four cytokines, TNF-α, IL-1α, IL-6 and IL-17, and monitored their impact on global gene expression by DNA microarray. The cytokines-supplemented substitutes have a more irregular epidermis, with protuberances and much thinner areas. Most interestingly, gene profiling on microarrays identified several genes reported as being deregulated psoriasis skin in vivo. Indeed, expression of the S100A12, IL8, DEFB4A and KYNU genes increased dramatically compared to their level in normal skin substitutes (P <0.005 to <0.05). In addition, the ACSBG1 gene, reported to be repressed in psoriasis, was also repressed in the cytokines-supplemented psoriatic substitutes compared to the controls (P <0.005). The product encoded by the genes deregulated in the cytokines-supplemented substitutes belong to biological pathways, such as the inflammatory and the immune responses, that are similarly altered in psoriasis in vivo. In conclusion, addition of cytokines to involved psoriatic substitutes alters the transcriptome of these cells in a manner similar to that observed with psoriasis in vivo. The addition of this pro-inflammatory cocktail, comparable cytokine in vivo psoriasis, prepares us for the next step: the characterization of the model once added immune cells.
Overall design Tissue-engineered psoriatic human skin (TEPHS) cultivated with (number of replicates: 3) or without (number of replicates: 3) Cytokines (IL-17a, IL-6, IL1a, TNF-a).
Contributor(s) Pouliot-Bérubé C, Zaniolo K, Soucy J, Guérin SL, Pouliot R
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Submission date Apr 10, 2015
Last update date Nov 27, 2018
Contact name Karine Zaniolo
Organization name Centre de recherche du CHU de Québec
Department CUO-Recherche
Lab Sylvain Guérin
Street address 1050 Chemin Sainte-Foy
City Québec
State/province Québec
ZIP/Postal code G1S 4L8
Country Canada
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (6)
GSM1655418 Tissue_engineered_Psoriatic_human_skin_control_rep1
GSM1655419 Tissue_engineered_Psoriatic_human_skin_control_rep2
GSM1655420 Tissue_engineered_Psoriatic_human_skin_control_rep3
BioProject PRJNA280856

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67762_RAW.tar 79.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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