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Series GSE67525 Query DataSets for GSE67525
Status Public on Apr 03, 2015
Title Molecular anatomy of palate development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Background: The FACEBASE consortium was established in part to create a central resource for craniofacial researchers. One purpose is to provide a molecular anatomy of craniofacial development. To this end we have used a combination of laser capture microdissection and RNA-Seq to define the gene expression programs driving development of the murine palate.
Results: We focused on the E14.5 palate, soon after medial fusion of the two palatal shelves. The palate was divided into multiple compartments, including medial and lateral, as well as oral and nasal, for both the anterior and posterior domains. A total of 25 RNA-Seq datasets were generated. The results provide a comprehensive view of the region specific expression of all transcription factors, growth factors and receptors. Paracrine interactions can be inferred from flanking compartment growth factor/receptor expression patterns. The results are validated primarily through very high concordance with extensive previously published gene expression data for the developing palate. In addition selected immunostain validations were carried out.
Conclusions: This report provides an RNA-Seq based atlas of gene expression patterns driving palate development at microanatomic resolution. This FACEBASE resource is designed to fuel discovery by the craniofacial research community.
Overall design Laser capture microdissection and RNA-seq were used to generate gene expression profiles of different compartments of the mouse E14.5 developing palate
Contributor(s) Potter AS, Potter SS
Citation(s) 26168040
Submission date Apr 02, 2015
Last update date May 15, 2019
Contact name Steven Potter
Organization name Children's hospital Medical Center
Department Developmental Biology
Lab Potter
Street address 3333 Burnet Ave.
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (25)
GSM1648770 E14.5 Ant. Lateral Rep. 1
GSM1648771 E14.5 Ant. Lateral Rep. 2
GSM1648772 E14.5 Ant. Lateral Rep. 3
BioProject PRJNA280162
SRA SRP056819

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Supplementary file Size Download File type/resource
GSE67525_RNA-seq_processed_data.txt.gz 3.6 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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