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Series GSE6751 Query DataSets for GSE6751
Status Public on Dec 31, 2007
Title Expression profiles of peripheral blood monocytes in periodontal therapy
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Periodontal infections have been associated with systemic inflammation and risk for atherosclerosis and vascular disease. We investigated the effects of comprehensive periodontal therapy on gene expression of peripheral blood monocytes. Approximately 1/3 of the patients showed substantial changes in expression in genes relevant to innate immunity, apoptosis, and cell signaling. We concluded that periodontal therapy may alter monocytic gene expression in a manner consistent with a systemic anti-inflammatory effect.
Keywords: time course,disease state analysis
 
Overall design Fifteen patients with periodontitis contributed with blood samples at four time points: 1 week prior to periodontal treatment (#1), at treatment initiation (baseline, #2), 6 weeks (#3) and 10 weeks post-baseline (#4). At baseline and 10 weeks, periodontal status was recorded and subgingival plaque samples were collected and processed by checkerboard DNA-DNA hybridization. Periodontal therapy, including periodontal surgery and extractions but no adjunctive antibiotics, was completed within 6 weeks. At each of the four time points, serum concentrations of 19 biomarkers were determined using multiplex assays for Luminex technology. Peripheral blood monocytes were purified, RNA was extracted, reverse-transcribed, labeled, and hybridized with Affymetrix U133 Plus 2.0 chips. Expression profiles were analyzed using linear random effects models. Further analysis of Gene Ontology (GO) terms summarized the expression patterns into biologically relevant categories. Treatment resulted in substantial improvement in clinical periodontal status and reduction in levels of several periodontal pathogens. Expression profiling over time revealed more than 11,000 probes sets differentially expressed at a false discovery rate of <0.05.
 
Contributor(s) Pavlidis P, Papapanou PN, Demmer R
Citation(s) 17716309
Submission date Jan 16, 2007
Last update date Mar 25, 2019
Contact name Paul Pavlidis
E-mail(s) paul@chibi.ubc.ca
Organization name University of British Columbia
Department Centre for High-Throughput Biology / Psychiatry
Street address 177 Michael Smith Laboratories 2185 East Mall
City Vancouver
State/province British Columbia
ZIP/Postal code V6T 1Z4
Country Canada
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (59)
GSM155448 Patient 08, time point 1
GSM155449 Patient 08, time point 2
GSM155450 Patient 08, time point 3
Relations
BioProject PRJNA98955

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Supplementary file Size Download File type/resource
GSE6751_RAW.tar 305.7 Mb (http)(custom) TAR (of CEL)

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