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Series GSE67437 Query DataSets for GSE67437
Status Public on Jul 28, 2015
Title Transcription factor Bcl11b controls identity and function of mature innate lymphoid cells type II
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Type-2 innate lymphoid cells (ILC2s) promote anti-helminth responses and contribute to allergies. Though Bcl11b has been previously considered a T-lineage identity transcription factor (TF) that restrains the innate-cell genetic programs, we report here that Bcl11b is highly expressed in mature ILC2s and acts upstream of the key ILC2 TFs Gfi1, Gata-3, and of IL-33 receptor IL1rl1 (T1ST2). Additionally, Bcl11b-/- ILC2s de-repressed RorĪ³t, Ahr and IL-23 receptor, normally expressed in type-3 ILCs (ILC3s). Consequently, Bcl11b-/- ILC2s lost ILC2 functions and gained ILC3 functions, expanding in response to the protease allergen papain, however producing IL-17 and IL-22, and not IL-5 and IL-13, causing lung neutrophilia rather than eosinophilia, and diminished mucus production. Our results broaden Bcl11b's role from a T-cell only TF, and establishes that Bcl11b sustains mature ILC2 genetic and functional programs and lineage fidelity through positive regulation of essential ILC2 TFs and negative regulation of pivotal ILC3 TFs.
 
Overall design RNA-seq analysis on sorted ILC2s from the mLNs of Bcl11bF/F Cre-ERT2 and wildtype mice at steady state following tamoxifen mediated deletion of Bcl11b
 
Contributor(s) Califano D, Avram D
Citation(s) 26231117
Submission date Mar 30, 2015
Last update date Jul 29, 2019
Contact name dorina avram
E-mail(s) avramd@mail.amc.edu
Phone 518-262-6731
Organization name albany medical college
Department center for immunology and microbial disease
Street address 47 new scotland ave
City albany
ZIP/Postal code 12208
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (2)
GSM1646799 TMX-Wild type control
GSM1646800 TMX-Bcl11bF/F Cre-ERT2
Relations
BioProject PRJNA279899
SRA SRP056712

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67437_KO-ILC2-mLN_021815_vs_WT-ILC2-mLN_021815.txt.gz 1.1 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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