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Series GSE67403 Query DataSets for GSE67403
Status Public on Jan 05, 2016
Title Spatial gene expression gradients underlie prominent heterogeneity of CA1 pyramidal neurons
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Tissue and organ function has been conventionally understood in terms of the interactions among discrete and homogeneous cell types. This approach has proven difficult in neuroscience due to the marked diversity across different neuron classes, but may also be further hampered by prominent within-class variability. Here, we considered a well-defined, canonical neuronal population – hippocampal CA1 pyramidal cells – and systematically examined the extent and spatial rules of transcriptional heterogeneity. Using next-generation RNA sequencing, we identified striking variability in CA1 PCs, such that the differences along the dorsal-ventral axis rivaled differences across distinct pyramidal neuron classes. This variability emerged from a spectrum of continuous expression gradients, producing a profile consistent with a multifarious continuum of cells. This work reveals an unexpected amount of variability within a canonical and narrowly defined neuronal population and suggests that continuous, within-class heterogeneity may be an important feature of neural circuits.
Overall design Hippocampal RNA profiles were generated by deep sequencing on Illumina HiSeq 2500, with three biological replicates per population
Contributor(s) Cembrowski MS, Spruston N
Citation(s) 26777276
Submission date Mar 30, 2015
Last update date May 15, 2019
Contact name Mark Steven Cembrowski
Organization name University of British Columbia
Department Cellular and Physiological Sciences
Lab Cembrowski
Street address 2350 Health Sciences Mall
City Vancouver
State/province BC
ZIP/Postal code V6S0B7
Country Canada
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (48)
GSM1867239 dorsal_0
GSM1867240 dorsal_1
GSM1867241 dorsal_2
BioProject PRJNA279837
SRA SRP056666

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67403_gene_exp.diff.gz 139.8 Mb (ftp)(http) DIFF
GSE67403_genes.fpkm_tracking.gz 4.2 Mb (ftp)(http) FPKM_TRACKING
GSE67403_genes.read_group_tracking.txt.gz 14.5 Mb (ftp)(http) TXT
GSE67403_mergedCount.txt.gz 1.4 Mb (ftp)(http) TXT
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Processed data are available on Series record
Raw data are available in SRA

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