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Series GSE6740 Query DataSets for GSE6740
Status Public on Feb 23, 2007
Title Comparison of transcriptional profiles of CD4+ and CD8+ T cells from HIV-infected pateints and uninfected control group
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We examined the gene expression profiles in ex vivo human CD4+ and CD8+ T cells from untreated HIV-infected individuals at different clinical stages and rates of disease progression. Profiles of pure CD4+ and CD8+ T cells subsets from HIV-infected nonprogressors who controlled viremia were indistinguishable from HIV-uninfected individuals. Similarly, no gene clusters could distinguish T cells from individuals with early from chronic progressive HIV infection, whereas differences were observed between uninfected or nonprogressors versus early or chronic progressors. In early/chronic HIV infection, three characteristic gene expression signatures were observed: (1) CD4+ and CD8+ T cells showed increased expression of interferon stimulated genes (ISGs). However, some ISGs including CXCL9, CXCL10, and CXCL11, and the IL15Rα in both CD4+ and CD8+ T cells and the anti-HIV ISG APOBEC3G in CD4+ T cells, were not upregulated. (2) CD4+ and CD8+ T cells showed a cluster similar to that observed in thymocytes, and (3) more genes were differentially regulated in CD8+ T cells than in CD4+ T cells, including a cluster of genes downregulated exclusively in CD8+ T cells. In conclusion, HIV infection induces a persistent T cell transcriptional profile, early in infection, characterized by a dramatic but potentially aberrant interferon response, and a profile suggesting an active thymic output.
Keywords: disease state analysis
Overall design We studied a cohort of HIV infected individuals with various clinical stages of HIV infection and healthy uninfected volunteers as a control group (Table 1). We included 5 individuals with early HIV infection (A), five with chronic progressive HIV infection (C), five individuals with non-progressive HIV infection with low or undetectable viral loads (L) and five HIV uninfected individuals (N). The HIV infected individuals were never on therapy prior to entering the study. Samples were taken once from each donor.
Contributor(s) Hyrcza MD, Ostrowski M, Der SD, Wilkins O, Yang S, Heisler L, Covacs C, Halpenny R, Loufty M
Citation(s) 17251300
Submission date Jan 12, 2007
Last update date Aug 10, 2018
Contact name Martin Hyrcza
Organization name University of Toronto
Lab Der/Ostrowski
Street address 1 King's College Circle, Rm 6356
City Toronto
State/province Ontario
ZIP/Postal code M5S1A8
Country Canada
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (40)
GSM154936 Human Acute HIV Sample A044-CD4 T cells
GSM155179 Human Acute HIV Sample A044-CD8+ T cells
GSM155180 Human Acute HIV Sample A051-CD4+ T cells
BioProject PRJNA98975

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Supplementary file Size Download File type/resource
GSE6740_RAW.tar 119.5 Mb (http)(custom) TAR (of CEL)

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