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Series GSE67171 Query DataSets for GSE67171
Status Public on Mar 18, 2017
Title Precocious Interleukin 21 expression in naïve mice defines a novel stage of T-follicular helper cell development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To define early forming IL21-expressing CD4+ T cells at the transcriptional level, we performed RNAseq on FACS-purified splenic CD4+ T cells from naïve 4 wk old IL21-VFP reporter mice based on the following criteria: naïve ICOSlo/- VFP- (N); activated ICOShi VFP- cells not expressing IL21 (ACT); and activated IL21-expressing cells ICOShi VFP+ cells (ACT IL21)
 
Overall design mRNA profiles of FACS-purified VFP-ICOS-, VFP-ICOS+, and VFP+ICOS+ CD4+ cell pools from naïve 4 wk old IL21-VFP reporter mice were generated by deep sequencing, in duplicate, using Illumina MiSeq
 
Contributor(s) Adkins EB, Wang X, Sproule TJ, Park G, Christianson GJ, Lane-Reticker SK, Carter GW, Morse III HC, Roopenian DC
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Submission date Mar 23, 2015
Last update date May 15, 2019
Contact name Xulong Wang
E-mail(s) xulong.wang@jax.org
Organization name The Jackson Laboratory
Street address 600 Main Street
City Bar Harbor
ZIP/Postal code 04609
Country USA
 
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (6)
GSM1641187 N rep1
GSM1641188 N rep2
GSM1641189 ACT rep1
Relations
BioProject PRJNA279169
SRA SRP056430

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67171_genes_TPM.txt.gz 353.3 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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