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Series GSE67171 Query DataSets for GSE67171
Status Public on Mar 18, 2017
Title Precocious Interleukin 21 expression in naïve mice defines a novel stage of T-follicular helper cell development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To define early forming IL21-expressing CD4+ T cells at the transcriptional level, we performed RNAseq on FACS-purified splenic CD4+ T cells from naïve 4 wk old IL21-VFP reporter mice based on the following criteria: naïve ICOSlo/- VFP- (N); activated ICOShi VFP- cells not expressing IL21 (ACT); and activated IL21-expressing cells ICOShi VFP+ cells (ACT IL21)
Overall design mRNA profiles of FACS-purified VFP-ICOS-, VFP-ICOS+, and VFP+ICOS+ CD4+ cell pools from naïve 4 wk old IL21-VFP reporter mice were generated by deep sequencing, in duplicate, using Illumina MiSeq
Contributor(s) Adkins EB, Wang X, Sproule TJ, Park G, Christianson GJ, Lane-Reticker SK, Carter GW, Morse III HC, Roopenian DC
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Submission date Mar 23, 2015
Last update date May 15, 2019
Contact name Xulong Wang
Organization name The Jackson Laboratory
Street address 600 Main Street
City Bar Harbor
ZIP/Postal code 04609
Country USA
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (6)
GSM1641187 N rep1
GSM1641188 N rep2
GSM1641189 ACT rep1
BioProject PRJNA279169
SRA SRP056430

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67171_genes_TPM.txt.gz 353.3 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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