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Series GSE66931 Query DataSets for GSE66931
Status Public on Nov 03, 2015
Title Single cell RNA-Seq of Hira-KO and control MII oocytes [Gdf9-Cre+]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The integrity of chromatin, which provides a dynamic template for all DNA related processes in eukaryotes, is maintained through replication dependent and independent assembly pathways. To address the role of replication independent histone deposition, we deleted the histone H3.3 chaperone Hira in developing mouse oocytes. We show that chromatin of non-replicative developing oocytes is highly dynamic, and that lack of continuous H3.3/H4 deposition alters chromatin structure, resulting in increased DNase I sensitivity, the accumulation of DNA damage, and, ultimately, a severe fertility phenotype. On the molecular level, abnormal chromatin structure leads to a dramatic decrease in the dynamic range of gene expression, the appearance of spurious transcripts, and inefficient de novo DNA methylation. In contrast to the only minor transcriptional phenotype observed in mouse pluripotent cells, we unequivocally show the importance of histone replacement and chromatin homeostasis for transcriptional regulation and normal developmental progression in an in vivo context.
Overall design RNA-Seq on 4 Hiraf/f and 4 Hiraf/f, Gdf9-Cre+ single MII oocytes
Contributor(s) Nashun B, Hill P, Dharmalingam G, Hajkova P
Citation(s) 26549683
Submission date Mar 16, 2015
Last update date May 15, 2019
Contact name Gopuraja Dharmalingam
Organization name MRC London Institute of Medical Sciences
Department Epigenetics section
Street address Faculty of Medicine, Imperial College, Hammersmith Hospital Campus
City London
ZIP/Postal code W12 0NN
Country United Kingdom
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (8)
GSM1634463 Hira-WT1_RNA-Seq [Gdf9-Cre+]
GSM1634464 Hira-WT2_RNA-Seq [Gdf9-Cre+]
GSM1634465 Hira-WT4_RNA-Seq [Gdf9-Cre+]
This SubSeries is part of SuperSeries:
GSE73382 Single cell RNA-Seq of Hira-KO and control MII oocytes
BioProject PRJNA278394
SRA SRP056202

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66931_HiraKO_FPKM.xlsx.gz 2.5 Mb (ftp)(http) XLSX
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Processed data are available on Series record
Raw data are available in SRA

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