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Series GSE66899 Query DataSets for GSE66899
Status Public on Jun 08, 2015
Title Batf3 maintains Irf8 autoactivation for commitment of a novel clonogenic progenitor of CD8+DCs
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The transcription factors Batf3 and IRF8 are required for development of CD8α+ conventional dendritic cells (cDCs), but the basis for their actions was unclear. Here, we identify two novel Zbtb46+ progenitors that separately generate CD8α+ and CD4+ cDCs and arise directly from the common DC progenitor (CDP). Irf8 expression in the CDP depends on prior PU.1-dependent autoactivation, and specification of pre-CD8 DC progenitors requires IRF8 but not Batf3. However, upon pre-CD8 DC specification, Irf8 autoactivation becomes Batf3-dependent at a CD8α+ cDC-specific enhancer containing multiple AP1-IRF composite elements (AICEs) within the Irf8 superenhancer. CDPs from Batf3-/- mice that specify toward pre-CD8 DCs fail to complete CD8α+ cDC development due to decay of Irf8 autoactivation, and divert to the CD4+ cDC lineage.
Overall design Examination of histone modifications (H3K27ac and H3K4me1) and 2 transcription factors (Batf3 and Irf8) and the p300 co-factor binding in 3 different dendritic cell subsets
Contributor(s) Iwata A, Murphy KM
Citation(s) 26054719
Submission date Mar 13, 2015
Last update date May 15, 2019
Contact name Gary E Grajales-Reyes
Organization name Washington University in St. Louis
Department Immunology
Lab Dr. Kenneth Murphy
Street address Room 7766, CSRB
City St Louis
State/province Mo
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (14)
GSM1633892 Batf3_CD24DC
GSM1633893 Irf8_CD24DC
GSM1633894 p300_CD24DC
BioProject PRJNA278283
SRA SRP056164

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Supplementary file Size Download File type/resource
GSE66899_RAW.tar 571.6 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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