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Series GSE66832 Query DataSets for GSE66832
Status Public on Nov 21, 2016
Title TET proteins regulate lineage specification and TCR-mediated expansion of iNKT cells (RNA-Seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary TET proteins oxidize 5-methylcytosine to 5-hydroxymethylcytosine and further oxidation products in DNA. Here we report that simultaneous deletion of Tet2 and Tet3 in mouse double-positive thymocytes resulted in dysregulated development and proliferation of invariant natural killer T (iNKT) cells. Tet2-Tet3-double-deficient (DKO) iNKT cells displayed pronounced skewing towards the NKT17 lineage, with increased DNA methylation and impaired expression of genes encoding the key lineage-specifying factors T-bet and ThPOK. Transfer of purified Tet2-Tet3 DKO iNKT cells into immunocompetent recipient mice resulted in uncontrolled expansion dependent on the nonclassical MHC protein CD1d, which presents lipid antigens to iNKT cells. Our data indicate that TET proteins regulate iNKT cell fate by ensuring proper development and maturation and suppressing aberrant T cell antigen receptor (TCR)-mediated proliferation.
Overall design DKO vs. wild type
Contributor(s) Tsagaratou A, Rao A
Citation(s) 27869820
Submission date Mar 12, 2015
Last update date May 15, 2019
Contact name Sini Rautio
Organization name Aalto University
Street address Konemiehentie 2
City Espoo
ZIP/Postal code 02100
Country Finland
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (19)
GSM1632848 iNKT WT BR1 RNA-Seq thymic iNKT
GSM1632849 iNKT WT BR2 RNA-Seq thymic iNKT
GSM1632850 iNKT WT BR3 RNA-Seq thymic iNKT
This SubSeries is part of SuperSeries:
GSE66834 TET proteins regulate lineage specification and TCR-mediated expansion of iNKT cells
SRA SRP056118
BioProject PRJNA282753

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66832_RAW.tar 3.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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