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Series GSE66741 Query DataSets for GSE66741
Status Public on Aug 01, 2015
Title RNA-sequencing in OS-RC-2 cells under the knockdown of Arkadia or ESRP2
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Tumor-specific alternative splicing is implicated in the progression of cancer, including clear cell renal cell carcinoma (ccRCC). Using ccRCC RNA-sequencing data from The Cancer Genome Atlas, we found that epithelial splicing regulatory protein 2 (ESRP2), one of the key regulators of alternative splicing in epithelial cells, is expressed in ccRCC. ESRP2 mRNA expression did not correlate with the overall survival rate of ccRCC patients, but the expression of some ESRP-target exons correlated with the good prognosis and with the expression of Arkadia (also known as RNF111) in ccRCC. Arkadia physically interacted with ESRP2, induced polyubiquitination, and modulated its splicing function. Arkadia and ESRP2 suppressed ccRCC tumor growth in a coordinated manner. Lower expression of Arkadia correlated with advanced tumor stages and poor outcomes in ccRCC patients. This study thus reveals a novel tumor-suppressive role of the Arkadia-ESRP2 axis in ccRCC.
Overall design Expression of mRNA in a ccRCC cell line OS-RC-2 under the knockdown of Arkadia or ESRP2. Knock-down of ESRP2 was confirmed by RT-PCR because of low expression of ESRP2 which resulted in non-quantitative FPKM value.
Contributor(s) Mizutani A, Koinuma D, Seimiya H, Miyazono K
Citation(s) 26522722
Submission date Mar 10, 2015
Last update date May 15, 2019
Contact name Daizo Koinuma
Organization name University of Tokyo
Department Pathology
Street address Hongo 7-3-1, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0033
Country Japan
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (3)
GSM1631243 RNA-seq, si Negative control OS-RC-2
GSM1631244 RNA-seq, si Arkadia OS-RC-2
GSM1631245 RNA-seq, si ESRP2 OS-RC-2
BioProject PRJNA277837
SRA SRP056037

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Supplementary file Size Download File type/resource
GSE66741_genes.fpkm_tracking.txt.gz 1.2 Mb (ftp)(http) TXT
GSE66741_isoforms.fpkm_tracking.txt.gz 2.0 Mb (ftp)(http) TXT
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