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Series GSE66723 Query DataSets for GSE66723
Status Public on Mar 11, 2015
Title Highly efficient in vivo delivery of functional RNAs using new versatile MS2-chimeric retrovirus-like particles
Organism Homo sapiens
Experiment type Expression profiling by array
Summary RNA delivery is a method of choice to achieve transient gene expression for research and in cell- or gene-based therapies. To improve retroviral transfer, we designed a dimerization-independent MS2-driven packaging system using MS2-retrovirus chimeras. We delivered RUNX2- or DLX5-mRNA into primary human bone-marrow mesenchymal-stem-cells.
We used microarrays to detail the global programme of gene expression confirming the effects of pro-osteogenic genes transduced by MS2 chimeric lentiviral particles.
Overall design Immature bone marrow mesenchymal stem cells were transduced with viral particles containing either MS2RLP-DLX5 or MS2RLP-RUNX2 or IDLV-Luc as control. Thirty hours after the first transduction, cells were transduced again as above. At 58 h, cells were washed 3 times with PBS and harvested. mRNA were then extracted.

Please note that data analyses were done in 3 groups;
Contributor(s) Prel A, Deschaseaux F, Pagès J
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Submission date Mar 10, 2015
Last update date Nov 08, 2016
Contact name Anne PREL
Organization name CNRS 5273 INSERM1031
Department Stromalab
Street address 1 Avenue J Poulhes
City Toulouse
ZIP/Postal code 31432
Country France
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (24)
GSM1630700 MSCs transduced with MS2RLP DLX5 - rep 1
GSM1630701 MSCs transduced with MS2RLP DLX5 - rep 2
GSM1630702 MSCs transduced with MS2RLP DLX5 - rep 3
BioProject PRJNA277806

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Supplementary file Size Download File type/resource
GSE66723_RAW.tar 91.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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