|
Status |
Public on May 20, 2015 |
Title |
Drop-Seq analysis of ERCC spike in capture |
Organism |
synthetic construct |
Experiment type |
Expression profiling by high throughput sequencing Other
|
Summary |
A bead supsension and a solution of ERCC spike-ins at a concentration of ~100,000 molecules per droplet was used in Drop-Seq, a novel technology for high-throughput single cell mRNAseq
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Overall design |
An estimated 84 beads were selected for amplification.
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|
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Contributor(s) |
Macosko EZ |
Citation(s) |
26000488, 32888429 |
|
Submission date |
Mar 09, 2015 |
Last update date |
Apr 25, 2022 |
Contact name |
Evan Macosko |
E-mail(s) |
emacosko@genetics.med.harvard.edu
|
Organization name |
Harvard Medical School
|
Department |
Genetics
|
Lab |
McCarroll Lab
|
Street address |
77 Avenue Louis Pasteur, NRB 260
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platforms (1) |
GPL19424 |
Illumina NextSeq 500 (synthetic construct) |
|
Samples (1) |
GSM1629193 |
ERCC at high sequencing depth (84 beads) |
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This SubSeries is part of SuperSeries: |
GSE63473 |
Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets |
|
Relations |
BioProject |
PRJNA277735 |
SRA |
SRP056001 |