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Series GSE66674 Query DataSets for GSE66674
Status Public on Mar 10, 2015
Title Identification of genes regulated during passaging of mouse osteosarcoma cell lines
Organism Mus musculus
Experiment type Expression profiling by array
Summary To identify the genes that are regulated during passaging of the parental mouse Dunn osteosarcoma cells with low metastatic potential and Dunn-derived LM8 cells with increased metastatic potential, we performed global gene expression profiling in early and late passage cells.
 
Overall design Total RNA was extracted from early and late passage cells cultured in three independent replicates for each condition. Cy3-labeled cRNA was synthesized and hybridized to Agilent mouse 4 x 44K microarrays. Raw data, obtained after array image analysis were normalized and used to retrieve differentially expressed genes in early and late passage cells.
 
Contributor(s) Muff R, Rath P, Ram Kumar RM, Lecca MR, Husmann K, Born W, Baudis M, Fuchs B
Citation(s) 25992885
Submission date Mar 09, 2015
Last update date Jun 10, 2019
Contact name Ram Mohan Ram Kumar
E-mail(s) rkumar@research.balgrist.ch
Organization name University Hospital Balgrist, University of Zurich, Zurich, Switzerland
Department Orthopaedics
Street address Forchstrasse 340
City Zurich
ZIP/Postal code 8008
Country Switzerland
 
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (12)
GSM1627728 Dunn_25_1
GSM1627729 Dunn_86_1
GSM1627730 LM8_24_1
This SubSeries is part of SuperSeries:
GSE66675 Identification of genes regulated during passaging of human and mouse osteosarcoma cell lines
Relations
BioProject PRJNA277711

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66674_Normalized_data_with_probename.txt.gz 1.7 Mb (ftp)(http) TXT
GSE66674_RAW.tar 32.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data are available on Series record

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